中国山核桃属植物种间亲缘关系RAPD分析

RAPD Analysis on Genetic Relationship among Species in Genus Carya

  • 摘要: 用随机引物扩增多态性DNA(RAPD)技术分析了山核桃属6个种及近缘属化香的种间亲缘关系,从600个随机引物中筛选出20个10bp多态性好的随机引物,通过扩增共得到317个DNA片段,片段大小为200~2800bp,其中多态性谱带为271条,占85.5%,表现出丰富的RAPD多态性;根据遗传距离,利用UPGMA构建品种亲缘关系树状图,结果表明RAPD分析的亲缘聚类基本上与经典分类相一致;从分子角度进一步证明大别山山核桃分种成立,与湖南山核桃、山核桃亲缘关系较近。

     

    Abstract: Genetic relationship among 6 species of genus Carya and species Platycarya strobilacea was analyzed by using RAPD analysis method.Twenty primers,which showed rich polymorphism,were screened out of 600 random primers,and totally 317 DNA fragments ranging from 200 to 2 800 bp were amplified,271 of these bands accounting for 85.5% were polymorphic.According to the genetic distances calculated by using UPGMA,the interspecies relationship dendrogram was constructed.It was indicated that the classification based on the genetic relationship among 6 species in genus Carya was basically consistent with the conventional classification.And the molecular evidences supported the opinion that C.dabieshanensis should be set aside as a new species,which was genetically close to C.hunanensis and C.cathayensis.

     

/

返回文章
返回