南方红豆杉愈伤组织培养研究

Studies on Callus Culture of Taxus chinensis var. mairei

摘要: 对南方红豆杉叶、茎的愈伤组织诱导条件及最适培养基组成成分进行了探讨.结果表明:2,4-D浓度2.0 mg/L比0.5 mg/L或1.0 mg/L处理的叶、茎愈伤组织诱导率高;嫩茎愈伤组织的诱导率因不同类型的激素组合以及激素不同质量浓度而不同,培养基MS+2,4-D 2.0 mg/L,MS+2,4-D 2.0 mg/L+6-BA 1.0 mg/L,MS+6-BA 1.0 mg/L+IAA 1.0 mg/L,1/2 MS+NAA 2.0 mg/L+IBA 1.0 mg/L,茎诱导率分别为100%,90%,75%,75%,相对其比较对象而言,这些培养基的诱导率最高.

Abstract: The induction condition and optimal medium constitution for callus growth from leaf and stem explants of Taxus chinensis var. mairei were studied. The results indicated that the callus induction frequency from young stems and leaves on the MS medium with 2.0 mg/L of 2,4-D was higher than those on the MS medium with 0.5 mg/L or 1.0 mg/L 2,4-D. The inducing frequency of callus from young stems varied with the combination of different plant growth regulators and different concentrations of the plant growth regulators. Compared to other combinations, the callus induction frequency from young stems as explants reached 100%, 90%, 75% and 75% respectively on the media MS+2,4-D 2.0 mg/L, MS+2,4-D 2.0 mg/L+6-BA 1.0 mg/L, MS+6-BA 1.0 mg/L+IAA 1.0 mg/L and 1/2 MS+NAA 2.0 mg/L+IBA 1.0 mg/L.