Abstract: To obtain flora distribution and characteristics of the Jialing River Wetland Nature Reserve in Sichuan Province, field investigation and related data access were developed in this study. The flora distribution of genera and species in the region was analyzed based on the criteria for seed plants distribution in China.The survey results show that there are 175 families, 730 genera and 1 700 species of vascular plants in the Jialing River Wetland Nature Reserve. And most belonged to seed plants, which is rich in biodiversity. Small families, as the dominant families of the reserve, were the main composition of ferns and seed plants in the flora, which accounted for 66.67% and 52.70% respectively. The flora of the nature reserve is complex, and the ferns are mainly cosmopolitan. The seed plants exhibit high tropical characteristics in the floristic composition of the family, and exhibit temperate nature in the genus composition. Moreover, the flora was characterized with certain Chinese peculiar elements, reflecting the zonality of vegetation in this region.