Abstract: The content of amino acids is an important index to measure the quality of bamboo shoots. The effects of main management measures such as the density of bamboo and fertilization on the content of amino acids of bamboo shoots were studied in order provide scientific basis for rational management of bamboo shoots forest. In this experiment, a two-factor randomized block design was adopted to determine the contents of 17 kinds of amino acids in bamboo shoots under different management measures. Three bamboo densities and three kinds of fertilization were set. The results showed that:(1) The density of bamboo had a significant effect on the amino acid content of bamboo shoots(P<0.05). The total contents of sweet amino acids, bitter amino acids, aromatic amino acids, human essential amino acids and free amino acids increased with the increase of bamboo density, but the total contents of umami amino acids were different and reached the maximum under the low bamboo density(2100～2550 plants /hm²).(2) There was no significant difference in amino acid content among different fertilization types. However, compared with the CK, application of organic fertilizer could significantly increase the content of aromatic amino acids and total free amino acids(P<0.05).(3) Among the different management measures, the amino acid content of bamboo shoots was higher when the bamboo density was 3300～3750 plants /hm² and applied with fertilizer. Compared with the CK, the content of bitter amino acids, aromatic amino acids and total free amino acids were significantly increased(P<0.05). In the management process of bamboo shoot forest, it can be considered to increase the amino acid content of bamboo shoots mainly by regulating the bamboo density.