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基于多点试验的白蜡全同胞家系遗传变异和选择
Genetic variation and selection of Full sibling family of Fraxinus based on multi-point experiments
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摘要: 以5年生不完全双列杂交获得的白蜡22个种内和种间全同胞家系为研究对象,通过株高和胸径等生长性状调查、方差分析和亲本间配合力计算,并结合遗传稳定性和适应性分析进行优良家系选择。结果表明:各家系间性状变幅幅度较大,最优家系平均值比最次家系高21.36%~73.32%;比亲本CK高5.78%~36.02%,家系生长性状在3地间存在交互效应;亲本鲁蜡6号、红2、金叶白蜡、红4、J30在株高方面优势明显,而亲本青碧、金叶白蜡、鲁蜡6号、红4和J30在胸径方面表现出优势;共筛选出株高优势组合2个、胸径优势组合3个,结合表型特征、速生性对优势组合进一步分析发现,‘杂交42号’‘杂交46号’ ‘杂交69号’和‘杂交70号’4个全同胞家系胸径和株高的表型值和杂种优势均显著或极显著的优于其他杂交组合;通过杂交组合基因型与区域环境互作效应及遗传稳定性、适应性分析发现,‘杂交69号’和‘杂交70号’稳定性较差,对特定区域有较强的适应性,其中‘杂交69号’在不同试验点分别表现出不同的优良性状,且整体抗逆性强。Abstract: In this study, 22 intra- and interspecific full-sib Fraxinus families, generated from incomplete diallel crosses and grown for five years across three sites in Shandong Province, were used as experiment materials. Superior family selection was conducted through investigation of growth traits including tree height and diameter at breast height, analysis of variance, calculation of parental combining ability, as well as integrated analysis of genetic stability and adaptability. The results revealed substantial variation in growth traits among all tested families. The mean trait values of the optimal families were 21.36% to 73.32% higher than those of the poorest performing families, and 5.78% to 36.02% higher than those of the parental control. A significant interaction effect on family growth traits was detected across the three test sites. General combining ability analysis showed that parental clones ‘Lu La 6’, ‘Hong 2’, ‘Jinyebaila’, ‘Hong 4’ and ‘J30’ exhibited prominent superiority in tree height, while parental clones ‘Qingbi’, ‘Jinyebaila’, ‘Lu La 6’, ‘Hong 4’ and ‘J30’ delivered superior performance in diameter at breast height. Specific combining ability analysis identified two hybrid combinations with superior tree height performance and three combinations with superior diameter at breast height performance. Further analysis of these superior combinations, integrating phenotypic characteristics and fast-growing properties, revealed that four full-sib families, namely ‘Hybrid 42’, ‘Hybrid 46’, ‘Hybrid 69’ and ‘Hybrid 70’, had significantly or extremely significantly higher phenotypic values and heterosis in both diameter at breast height and tree height relative to all other hybrid combinations. Analysis of genotype by regional environment interaction effect, genetic stability and adaptability of the hybrid combinations demonstrated that ‘Hybrid 69’ and ‘Hybrid 70’ had low stability but strong adaptability to specific regions. ‘Hybrid 69’ exhibited distinct superior traits at different test sites, and also presented strong overall stress resistance.
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