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百山祖不同海拔土壤酶活性与生态化学计量的变化特征及影响因素
Variations and Drivers of Soil Extracellular Enzyme Activities and Ecoenzymatic Stoichiometry Across Elevation Bands in Baishanzu National Park
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摘要: 以百山祖国家公园山地生态系统为研究对象,沿海拔梯度系统探讨土壤胞外酶活性及酶化学计量特征的变化规律,分析植物多样性和土壤理化因子对土壤胞外酶活性及其化学计量特征的相对影响。在700~900 m(常绿阔叶林)、
1100 ~1300 m(针叶阔叶混交林)、1300 ~1500 m(针叶林)和1500 ~1700 m(灌木林)4个海拔区间设置48个样地,调查植物多样性并采集0~20 cm表层土壤样品,测定土壤理化性质及5种参与碳(C)、氮(N)、磷(P)循环的胞外酶活性,包括β-1,4-葡萄糖苷酶(BG)、纤维二糖水解酶(CBH)、β-1,4-N-乙酰氨基葡萄糖苷酶(NAG)、L-亮氨酸氨基肽酶(LAP)和酸性磷酸酶(AP)。采用酶化学计量比、向量分析、Mantel检验和冗余分析揭示土壤酶活性及其调控因子。结果表明:不同海拔土壤胞外酶活性存在明显差异,BG、CBH、LAP和AP活性总体随海拔升高而增加,并在1500 ~1700 m达到最高值;NAG活性在1100 ~1300 m和1500 ~1700 m最高;胞外酶C/N在1100 ~1300 m最低,胞外酶C/P在700~900 m最低,胞外酶N/P在1100 ~1300 m最高;各海拔带向量长度(VL)整体随海拔升高而升高,向量角度(VA)则降低但均大于45°,表明低海拔P限制相对更强,高海拔C限制更强;土壤含水量(SM)和pH是不同海拔土壤酶活性及其化学计量特征的重要因子,土壤有机碳(SOC)、可溶性有机碳(DOC)和土壤总无机氮(TIN)等土壤理化因子也与酶活性变化密切相关,而植物多样性指标与土壤酶活性及其化学计量特征的相关性较弱。百山祖不同海拔区间土壤胞外酶活性及酶化学计量特征具有明显分异,土壤微生物表现出不同程度的C和P限制。Abstract: This study focused on carbon (C), nitrogen (N), and phosphorus (P) nutrient cycling in the montane ecosystem of Baishanzu National Park. We investigated variations in soil extracellular enzyme activities and ecoenzymatic stoichiometric characteristics along an elevational gradient and analyzed the relative effects of plant diversity and soil physicochemical factors on soil extracellular enzyme activities and their stoichiometric characteristics. A total of 48 plots were established across four elevation bands, including 700~900 m (evergreen broadleaved forest),1100 ~1300 m (coniferous and broadleaved mixed forest),1300 ~1500 m (coniferous forest), and1500 ~1700 m (shrubland). Plant diversity was surveyed, and surface soil samples from the 0~20 cm layer were collected. Soil physicochemical properties and the activities of five extracellular enzymes involved in C, N, and P cycling were measured, including β-1,4-glucosidase (BG), cellobiohydrolase (CBH), β-1,4-N-acetylglucosaminidase (NAG), L-leucine aminopeptidase (LAP), and acid phosphatase (AP). Ecoenzymatic stoichiometric ratios were calculated, and vector analysis, Mantel tests, and redundancy analysis were used to identify the major factors associated with soil extracellular enzyme activities and their stoichiometric characteristics. The results showed that soil extracellular enzyme activities differed markedly among elevation bands. The activities of BG, CBH, LAP, and AP generally increased with elevation and reached their highest values at1500 ~1700 m, whereas NAG activity was highest at1100 ~1300 m and1500 ~1700 m. The ecoenzymatic C/N ratio was lowest at1100 ~1300 m, the ecoenzymatic C/P ratio was lowest at 700~900 m, and the ecoenzymatic N/P ratio was highest at1100 ~1300 m. Vector analysis showed that vector length (VL) increased with elevation, whereas vector angle (VA) decreased but remained greater than 45° across all elevation bands, indicating stronger P limitation at lower elevations and stronger C limitation at higher elevations. Soil moisture content (SM) and pH were important factors associated with soil extracellular enzyme activities and their stoichiometric characteristics across elevation bands. Soil physicochemical factors such as soil organic carbon (SOC), dissolved organic carbon (DOC), and total inorganic nitrogen (TIN) were also closely related to changes in enzyme activities, whereas plant diversity indices showed relatively weak correlations with soil extracellular enzyme activities and their stoichiometric characteristics. Overall, soil extracellular enzyme activities and ecoenzymatic stoichiometry differed markedly among elevation bands in Baishanzu National Park, and soil microorganisms exhibited varying degrees of C and P limitation.
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