Preliminary Study on Propagation of Clematis patens

A research on propagation of Clematis patens was carried out for germplasm conservation.It was showed by the results that generative propagation was not feasible because of the low rate of seed germination.It was proven that the rooting process of cuttage could be promoted by soaking the cuttings in IAA solution with 0.637 m mol/L of concentration for 2 or 3 minutes, or by instant dipping the cuttings into IAA solution with 2.548 m mol/L of concentration prior to the cuttage operation.The result of tissue culture indicated that the formulae of (MS +6 -BA 2.0 mg/L +2, 4 -D 1.0 mg/L)and (MS +6 -BA 2.0mg/L +IBA 1.0 mg/L)were better at the stage of inducing calli when leaves and apical meristems were adopted as explants.The formula of (MS +6 -BA 2.0 mg/L +NAA 0.5 mg/L)was the best in the process of successive enrichment culture, and the formula of (1/2 MS +IAA 0.8 mg/L)was the best for root induction.