ZHAO Yang, CHEN Xiao-yang, LI Tong-sen, HU Dong-nan. Establishment and Optimization of ISSR ReactionSystem for Lespedeza spp.[J]. Journal of Southwest Forestry University, 2006, 26(2): 6-9,23. DOI: 10.11929/j.issn.2095-1914.2006.02.002
Citation: ZHAO Yang, CHEN Xiao-yang, LI Tong-sen, HU Dong-nan. Establishment and Optimization of ISSR ReactionSystem for Lespedeza spp.[J]. Journal of Southwest Forestry University, 2006, 26(2): 6-9,23. DOI: 10.11929/j.issn.2095-1914.2006.02.002
shu

Establishment and Optimization of ISSR Reaction
System for Lespedeza spp.

    Graphical Abstract
    • Abstract
  • The influencing factors including genomic DNA extraction, concentration of template DNA, dosage of Taq DNA polymerase, primer concentration, dNTP dosage and the annealing temperature upon the amplification effect with PCR technique were studied for optimizing the ISSR reaction conditions for studies on the genetic diversity of Lespedeza spp.germplasm resources. The results showed that the optimum reaction conditions for ISSR PCR of Lespedeza spp. were as follows:1.0 U Taq DNA(produced by TIANWEI company)+2 μL 10×Taq buffer (200 mM Tris-HCl; 100 mM(NH4)2SO4;200 mM KCl;15 mM MgCl2)+40 ng template DNA+0.2 m mol/L L dNTP+0.2 μmol/L primer.
    • FullText(HTML)
  • loading
    • References (0)
    • Related Articles

Catalog

    Turn off MathJax
    Article Contents

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return