On Peroxidase Isoenzymes of Plants in Genus of Keteleeria

Peroxidase isoenzymes of 8 species in genus of Keteleeria were analyzed by means of Disc polyacrylamide gel electrophoresis. It was showed that each species had its specific zymogram and the differences in zymograms among the species were obvious. The similarity of zymograms of the 8 species was studied by systematic clustering method applied to quantitative analysis. According to the zymograms, the 8 species might be classified into 3 groups and 6 species, by which 2 pairs of 2 species were merged respectively into 2 species. GroupⅠincluded Keteleeria fortunei and Keteleeria cyclolepis. Group Ⅱ contained Keteleeria davidiana and Keteleeria calcarea. The third group comprised Keteleeria evelyniana and Keteleeria xerophila. It was proposed that Keteleeria davidiana var. chien-peii should be merged with Keteleeria davidiana, and Keteleeria evelyniana var. pendula should be merged with Keteleeria evelyniana. It was deduced that Keteleeria xerophila might be an ecotype of Keteleeria evelyniana. The results from peroxidase isoenzyme experiment verified at molecular level the consanquineous relations among the species in genus of Keteleeria.