Induction and Multiplication Culture of Organogenetic Callus of Pinus caribaea

The optimum conditions for organogenetic callus induction and multiplication culture were studied for Pinus caribaea by taking the mature zygotic embryos,apical buds of embryo sprouts,hypocotyls and radicle as explants.Three media DCR,LP,TX with different concentration of NAA,2,4-D,6-BA plant growth regulators were applied.The results showed that the mature zygotic embryos and apical buds of the embryo sprouts were best explants for induction of organogenetic callus,and the best induction medium was TX+BA 0.5 mg/L+NAA 2.0 mg/L+30 g/L sucrose,the induction rate reached 50%.The best medium for multiplication culture was TX+BA 0.3 mg/L+NAA 1.0 mg/L+CH 400 mg/L+30 g/L sucrose,the successive transfer cycle was 25~30 d,and the multiplication rate was 3.46.