Construction of SOC1 Gene Expression Vector and Bioinformatic Analysis in Fraxinus mandschurica

To study the role of flowering regulation gene SOC1 in Fraxinus mandschurica, We cloned the fulllength coding region sequence of SOC1 from Fraxinus mandschurica. Sequence analysis showed that the gene is 654 bp in length, encoding a protein of 218 amino acid. The gene has the highest similarity with Antirrhinum majus MADSbox transcription factor DEFH68 gene, the nucleotide similarity is 78%. The gene has MADSbox domain, Kdomains, multiple DNA binding sites and phosphorylation sites, so it is a transcription factor of MADSbox family. In this study, we successfully constructed a Fraxinus mandschurica SOC1 gene plant expression vector pROKⅡ-35S∶∶SOC1 with the use of double digestion method, laying a solid foundation for the further genetic transformation in Fraxinus mandschurica.