Cloning and Analysis of UFGT Gene Sequence from Pyrus pyrifolia cv. Huobali of Specific in Yunnan

In order to research UFGT gene for regulation mechanism of anthocyan synthesis in red pear, Pyrus pyrifolia cv. Huobali of specific in Yunnan was used as experimental material and the cDNA fragment of UFGT gene was obtained by homologous cloning. Then, bioinformatics analysis of UFGT gene and its deduced protein was done by using the related biological software. The results showed that length of UFGT is 1 440 bp, and it encodes 479 amino acids. The homology at amino acids level of UFGT with UFGT in Pyrus pyrifolia (AFQ92055)、Pyrus communis (AGL81353)、Pyrus bretschneideri (AGZ15304)、Malus domestica (XP_008357063) and Prunus cerasifera (AKV89253) are 99%, 96%, 94%,91% and 72%, respectively. The results of amino acid sequence analysis indicated that the UFGT had no signal peptide and transmembrane domain, belonged to the non secreted protein and located in cytosol. The protein predication of UFGT showed that there were a function domains GT1_Gtf_Like and typical structure of GTB super family.