Establishment of Hairy Root Culture of Erigeron breviscapus

To establish the cultural system for hairy root of Erigeron breviscapus root of Ebreviscapus was obtained from leaf explants after infected with Agrobacterium rhizogenes C58C1 strains, determine the growth curve of hairy roots, and compared the effect of hairy roots increment by different factors. The results showed that the hairy root of Ebreviscapus was successfully induced by using Arhizogenes strain C58C1, and the best system of hairy root culture was established. The best concentration of Arhizogenes liquid is OD600=06, the best infection time is 10min, and the best coculture time is 2d. These conditions allow the inducement rate was 60%. The transformation of TDNA from Ri plasmid to the hairy root was confirmed by PCR analysis.