In vitro Regeneration System of 'Hort16A' Kiwifruit Introduced from Abroad

The tissue culture regeneration system of kiwifruit 'Hort16A' (Actinidia chinensis 'Hort16A') introduced from abroad was established. The callus formation rate and adventitious bud differentiation rate were investigated using theL₉(3⁴)orthogonal experiment, proliferation of adventitious buds were further studied using single factor randomized block design of experiment, and the rooting of kiwifruit was researched using theL₉(3⁴)orthogonal experiment. The result indicated that the highest callus formation rate (97.78%±1.92%) was investigated from the explants in the MS medium contained 0.05 mg/L NAA and 0.3 mg/L ZT after culturing 20 d. That the differentiation rate and height of adventitious buds were (84.44%±1.92%) and (1.52±0.29) cm respectively were the best differentiation effect surveyed on the explants in this medium after another 14 d culturing. The biggest proliferation rate (3.67±0.33) and height (2.93±0.12) cm of adventitious buds were surveyed on the buds in the MS medium contained 1.5 mg/L ZT after culturing 30 d. The best rooting that rooting ratio, root length, root width and number of roots generated per shoot were (98.9%±1.9%), (7.60±0.44) cm, (0.47±0.07) cm and (7.67±2.19) respectively was investigated from the shoots in the 1/2 MS medium contained 0.7 mg/L IBA and 0.1 g/L activated carbon.