Effects of Macroelement Concentration on Growth and Secondary Metabolites Contents of Polygonatum cyrtonema′s Adventitious Bud
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Abstract
Taken germinative rootstock of Polygonatum cyrtonema as the material. And the experiment was based on MS + NAA 1.0 mg/L+ KT 1.5 mg/L medium. It was investigated the complex effect that made by the concentration of the 5 kinds of macroelements in MS medium on the growth of germinative rootstock of P.cyrtonema and the 2 kinds of secondary metabolites include amylose and total saponins. The results indicated that in the most suitable medium for the growth of P.cyrtonema germinative rootstock, the concentration of NH4NO3 and KNO3 was 1.5 times as strong as the standard concentration of MS medium. The concentration of KH2PO4, MgSO4 and CaCl2 was 2 times as strong as the standard concentration of MS medium. In the most suitable medium that synthesized by total anylose, the concentration of KH2PO4 was 2 times as strong as the standard concentration of MS medium. In addition, the other elements were all as strong as the standard concentration of MS medium. In the most suitable medium that synthesized by total saponins, the concentration of 5 kinds of macroelements were MS medium′s standard concentration.
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