Identification of Pathogen Causing Coffee Tree Brown Leaf Spot and Disease Resistance of Coffea arabica Varieties in Field

Pathogen of coffee tree brown leaf spot were identified by means of isolation culture, pathogenicity test, and the combination of morphology and molecular biology identification.At the same time, the investigation of occurrence and varieties resistance on coffee brown spot disease were carried out.The results showed that morphological characteristics of 3 pathogenic strains from coffee leaf samples in different places were the same.CCYN05 and CCHN05 were purified, and 2 strains were the same pathogen by rDNA-ITS sequence alignment and analysis.And combined with morphology characteristics, the results revealed that this disease was caused by Cercospora coffeicola.The brown spot disease resistances of 5 Arabica coffee tree varieties were investigated in the field, there were no immune varieties and disease-resistant varieties.The disease resistances sequences were as following:Catuaí Regional, Caturra, Catuaí44, Catimor, Villa Sarchí.