The Transferability Analysis of Pinus kesiya var. langbianensis EST-SSR Markers to Several Pine Species

The transferability of 18 Pinus kesiya var. langbianensis EST-SSR markers to related species was studied. The transferability percentages of selected primers to P.massoniana, P.densata, P.tabuliformis and P.yunnanensis were 88.89%. The percentage of polymorphism of 18 EST-SSR markers to P.massoniana, P.densata, P.tabuliformis and P.yunnanensis were 81.25%, 81.25%, 75.00% and 93.75%, respectively. These results indicated that it was feasible to develop SSR markers from P.kesiya var. langbianensis transcriptome sequences. P.kesiya var. langbianensis EST-SSR markers was highly transferable and polymorphic to its related species. These markers can be used to analyze the genetic relationship between the P.kesiya var. langbianensis and its related species.