The Destructive Effects of 4 Species of Trichoderma on Cronartium ribicola

In order to know the ability of the genus Trichoderma producing cell-wall degrading enzyme as well as screening and obtaining fine biocontrol strains for the plant rust degradation, 48 strains of 4 species of Trichoderma were inoculated and moisturizingly cultured along with regularly observed on destructive effects. The results showed that among the 4 species, all strains of T.longibrachiatum grew slowly on the aeciospore piles of Cronartium ribicola without any destructive effects on aeciospore walls. However, lots of Trichoderma strains of the other 3 species could live on the aeciospore piles with significantly different growth rate and destructive capacity on aeciospores due to various Trichoderma species or strains of the same species. Among the 3 Trichoderma species, a majority of T.atroviride grew well on aeciospore piles and the walls of the aeciospores were strongly destroyed, following with some strains of T.koningii and T.harzianum. Besides, DNS-developing method was used to detect the 3 species of Trichoderma′s activities of producing cell-wall degrading enzyme (chitinase and β-1, 3-glucanase) induced by walls of aeciospores. The result manifested that their capacities of inducing enzyme activities basically agreed with the strains′ destructive effects on the inoculated aeciospores walls.