Process Optimization of Paraffin Section Method for Scale of Cunninghamia lanceolata

In order to accurately observe the cell internal structure of Cunninghamia lanceolata scales, the processes, which was summarized by comparing the results of different treatments including 2 different fixing solutions, 3 softening treatments, 4 dehydrant and transparent treatments, time and temperature of waxing, embedding and dyeing, were optimized. Results showed that the material could be both fixed effectively by the FAA and FPA solutions; the best softening process was the hydrochloric acid solution for 12 h; the best dehydration time was 2 h per level, and the best transparent time was 1 h per level. Clear and complete cell structure map of the scales of C.lanceolata could be obtained by using this method.