Establishment of Adventitious Root Culture System of Vaccinium dunalianum

Using the healthy leaves of tissue culture seedling of Vaccinium dunalianum as the material to directly establish the adventitious root culture system. The effects of different dark culture time, plant growth regulator concentration, carbon source and medium on the induction of adventitious root were investigated, and its induction condition was optimized by orthogonal design. The results showed that the optimal culture condition for leaf adventitious root induction was WPM + 1.0 mg/L IBA + 50.0 g/L sucrose with dark culture for 15 d, with a rooting rate of 96.7%.