Fengjuan Wen, Tingfeng Gong, Tingfeng Gong, Yuntao Li, Mingfu Zhao, Shaozhong Xu, Guosong Wen. Identification of Salvia yunnanensis and Relative[J]. Journal of Southwest Forestry University, 2018, 38(4): 29-36. DOI: 10.11929/j.issn.2095-1914.2018.04.005
Citation: Fengjuan Wen, Tingfeng Gong, Tingfeng Gong, Yuntao Li, Mingfu Zhao, Shaozhong Xu, Guosong Wen. Identification of Salvia yunnanensis and Relative[J]. Journal of Southwest Forestry University, 2018, 38(4): 29-36. DOI: 10.11929/j.issn.2095-1914.2018.04.005
shu

Identification of Salvia yunnanensis and Relative

    Graphical Abstract
    • Abstract
  • Materials from 8 locations areas were selected for the genomic DNA extraction, then ITS sequence was amplified and sequenced.Salvia yunnanensis and Saliva miltiorrhiza were identified and clustered by ITS barcode and its secondary structure.Sequence alignment were performed by DNAMAN 8.0, the NJ phylogenetic tree was built by MEGA 6.0, the secondary structure of ITS1 and ITS2 sequences were predicted by RNAstruct 6.0.The results of study show that there is a significant difference between ITS sequences of S.yunnanensis and S.miltiorrhiza, which is divided into 2 branches in the NJ phylogenetic tree, 5.8S rDNA sequences are basically the same and show a high degree of conservation among them.The secondary structure indicates that ITS1 and ITS2 regions show a large interspecific divergence.Therefore, the ITS sequence could provide a basis for the identification of S.yunnanensis, S.miltiorrhiza and other related species.
    • FullText(HTML)
  • loading
    • References (31)
    • Related Articles
  • Supplements (0)

Catalog

    Turn off MathJax
    Article Contents

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return