Gene Cloning and Expression Analysis of VdARP7 in Vaccinium dunalianum

The cDNA fragment of ARP7 gene was isolated by transcriptome sequencing method from leaf bud of Vaccinium dunalianum. The cDNA sequence of VdARP7 gene was cloned with RACE-PCR. Semi-quantitative PCR was used to analyze the expression of VdARP7 gene in different tissues of V. dunalianum. The findings indicate that gene VdARP7 belong to the Actin superfamily ARPs subfamily and had high homology with ARP7 genes from many other higher plants. The open reading frame encoded a putative C-terminal sequence with 225 amino acid residues of VdARP7 protein. The VdARP7 also had the NBD_sugar-kinase_HSP70_actin conserved domain. The VdARP7 mRNA was stably expressed in leaf bud, young leaf, old leaf, flower bud and flower of V. dunalianum.It is speculated that ARP7 as a constitutively expressed protein plays an important physiological functions in plant growth and development.