Optimization of SSR-PCR Reaction System in Pinus kesiya var. langbianensis

By using the genomic DNA of JG1 as template, an orthogonal design was employed to optimize SSR-PCR system of Pinus kesiya var. langbianensis, and the optimum SSR-PCR reaction system of P. kesiya var. langbianensis was established. The results showed that the optimum reaction system was 25 μL reaction solution containing Mg²⁺ 2.0 mmol/L, DNA 60 ng, primer 0.50 μmol/L, Taq DNA polymerase 0.25 U, dNTP 0.20 mmol/L. To verify the reaction system DNA of 11 individuals were amplified, the results indicated that the optimized reaction system was proved in good stability.