Construction and Identification of AtIDD4 and 3×Flag Fusion Protein Expressing in Arabidopsis thaliana

In order to study an interaction between transcription factor AtIDD4 and downstream DNA using chromatin immunoprecipitation (ChIP) technique, the prokaryotic expression vector of Agrobacterium tumefaciens 35S-AtIDD4CDS-3×Flag was constructed in this paper. And this structure was transferred into wild-type Arabidopsis thaliana to achieve co-expression and overexpression of the ATIDD4 and 3×Flag tag. The results showed that the expression of AtIDD4 and 3×Flag tag were examined by the methods of PCR amplification, DNA sequencing and quantitative RT-PCR, the AtIDD4 and 3×Flag were co-expressed. And the expression level of AtIDD4 was dramatic increased in transgenic plants.