Peihua Gan, Dan Li, Dezhou Shen, Anpei Zhou, Zhongli Xie, Chengzhong He. Analysis on Genetic Diversity and Genetic Structure of Different Floral Patterns of Camellia reticulate f. simplex[J]. Journal of Southwest Forestry University, 2019, 39(4): 46-52. DOI: 10.11929/j.swfu.201902066
Citation: Peihua Gan, Dan Li, Dezhou Shen, Anpei Zhou, Zhongli Xie, Chengzhong He. Analysis on Genetic Diversity and Genetic Structure of Different Floral Patterns of Camellia reticulate f. simplex[J]. Journal of Southwest Forestry University, 2019, 39(4): 46-52. DOI: 10.11929/j.swfu.201902066
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Analysis on Genetic Diversity and Genetic Structure of Different Floral Patterns of Camellia reticulate f. simplex

  • 1.

    Key Laboratory for Forest Genetic and Tree Improvement & Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming Yunnan 650233, China

  • 2.

    Key Laboratory of State Forestry Administration for Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming Yunnan 650233, China

  • 3.

    Yunnan Academy of Biodiversity, Southwest Forestry University, Kunming Yunnan 650233, China

  • 4.

    Key Laboratory of Ministry of Education for Forestry Resources Conservation and Utilization in the Southwest Mountains of China, Southwest Forestry University, Kunming Yunnan 650233, China

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  • Received Date: February 21, 2019
  • Revised Date: March 24, 2019
  • Available Online: April 25, 2019
  • Published Date: June 30, 2019
    Graphical Abstract
    • Abstract
  • Fluorescent AFLP markers were used to analyze the genetic varieties of 90 samples from 3 natural floral patterns of Camellia reticulate f. simplex collected from Tengchong County. The results showed that a total of 697 DNA fragments were produced by 7 AFLP primer combinations. At species level, all DNA fragments were found to be polymorphic (100%), effective number of alleles (Ne) was 1.361 4, Nei’s gene diversity (H) was 0.257 6, and Shannon’s information index was (I) 0.421 5. All of the genetic diversity parameters revealed that there was a relatively high level of genetic diversity in C. reticulate f. simplex. On the level of natural floral pattern, the scope of Hnfp and Infp was from 0.241 8 to 0.255 8 and from 0.395 9 to 0.413 2, respectively. The order of genetic diversity level was single floral pattern >semi-double floral pattern >double floral pattern. Genetic differentiation coefficient (Gst) among 3 natural floral patterns was 0.034 5, which was consistent with the result of AMOVA (Φst=0.056). It is suggested that higher degree of genetic varieties within natural floral pattern were the main source of genetic diversity in C. reticulate f. simplex, and close protective attention should be payed to individuals. The 3 natural floral patterns were divided into 2 groups by UPGMA analysis based on Nei’s genetic distances. Of which, one group was composed of simple floral pattern, and the other group was composed of semi-double floral pattern and double floral pattern. Furthermore, UPGMA clustering result showed that 90 individuals were divided into mainly 3 groups based on Nei’s genetic distances among individuals, and clearly separate the 3 natural types. This clustering result revealed that semi-double floral pattern was a transitional pattern between single floral pattern and double floral pattern, and the 3 natural floral patterns occupied their own unique genetic material basis. Therefore, it was scientific and reasonable that C. reticulate f. simplex was classified into 3 natural floral patterns.
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