Induction and Differentiation of Callus and Propagation of Adventitious Buds in Passiflora caerulea

Cotyledons of Passiflora caerulea were used as experimental materials to induce callus, differentiation and adventitious buds proliferation. The results showed that the callus induction medium using MS+2,4-D 1.0 mg/L+TDZ 0.5 mg/L+Agar 3.8 g/L+Sucrose 30 g/L had the highest rate of callus induction, and the induction rate was 73.33%. The optimum medium for inducing buds was MS+2,4-D 0.5 mg/L+TDZ 2.0 mg/L+Agar 3.8 g/L+Sucrose 30 g/L, the induction rate reached 46.67%.The optimum medium for further culturing adventitious buds was MS medium+6-BA 1.0 mg/L+IAA 0.3 mg/L+Agar 4.0 g/L+Sucrose 30 g/L, the multiplication multiple was 2.97. The established plant regeneration system could provide technical support for rapid propagation in vitro, ploidy breeding and genetic transformation of P. caerulea.