Study on the Influencing Factors of Bud Proliferation and Bulblet Swelling of Lilium fargesii in vitro

In order to optimize the factors affecting the bud proliferation and bulblet swelling of Lilium fargesii in vitro. The effects of sucrose concentration, hormone concentrations and sucrose combinations, concentrations of adenine sulphate and casein hydrolysate on the proliferation, and sucrose concentration, hormone concentrations and combinations on the swelling in vitro were investigated. The results showed that MS+TDZ 0.2 mg/L+NAA 0.2 mg/L+Sucrose 30 g/L+Adenine sulphate 30 mg/L+Casein hydrolysate 200 mg/L was suitable for bud proliferation from scales of L. fargesii in vitro under the condition of the culture temperature of (23±1) ℃ and light culture of 12h/d, and multiplication coefficient was 5.59; The optimal medium of bulblet swelling was MS+NAA 0.3 mg/L+6-BA 0.5 mg/L+ Sucrose 60 g/L. This study comes a conclusion that excessive or too low concentration of sucrose was to the disadvantage of the bud proliferation and swelling of test-tube bulblets of L. fargesii, the multiplication rate in light was higher than in dark, suitable combinations of casein hydrolysate played an auxoaction for the bud proliferation and bulblet swelling, and synergism of cytokinin and auxin can significantly promote the bud proliferation and bulblet swelling.