Selection and Validation of SRAP Primers in Pinus armandii

In order to screen primers for SRAP genetic diversity of Pinus armandii, young disease-free needles from 30 superior clones from 6 provenances of P. armandii clonal seed orchard were used as experimental materials. Based on SRAP marker technique, P. armandii DNA were amplified with 100 SRAP primers combinations combined randomly with 10 forward and 10 reverse primers. It was found that a total of 15 pairs of effective primers with rich polymorphism and high repeatability were screened. A total of 3 767 DNA bands were amplified by 15 pairs of primers. Among them, 2 448 polymorphic bands accounted for 64.99% of the total bands, with an average polymorphic rate of 64.28%. The 15 pairs of SRAP primers obtained from the experiment were suitable for the genetic diversity analysis of P. armandii.