Zhiduo Wen, Yong Ding, Weina Shang, Tiantian Wang, Shaozheng Wu, Xiaoxia Su, Jie Zhang, Zhongkai Zhang, Kuanyu Zheng. Distribution Characteristics of TSWV Virions in Vascular Tissues of the Systemic Host Tobacco K326[J]. Journal of Southwest Forestry University, 2020, 40(6): 124-131. DOI: 10.11929/j.swfu.202001020
Citation: Zhiduo Wen, Yong Ding, Weina Shang, Tiantian Wang, Shaozheng Wu, Xiaoxia Su, Jie Zhang, Zhongkai Zhang, Kuanyu Zheng. Distribution Characteristics of TSWV Virions in Vascular Tissues of the Systemic Host Tobacco K326[J]. Journal of Southwest Forestry University, 2020, 40(6): 124-131. DOI: 10.11929/j.swfu.202001020
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Distribution Characteristics of TSWV Virions in Vascular Tissues of the Systemic Host Tobacco K326

  • 1.

    Key Laboratory of Forest Biotechnology in Yunnan, Southwest Forestry University, Kunming Yunnan 650233, China

  • 2.

    Life Sciences Institute, Zhejiang University, Hangzhou Zhejiang 310058, China

  • 3.

    Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming Yunnan 650205, China

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  • Received Date: January 09, 2020
  • Revised Date: March 01, 2020
  • Available Online: June 23, 2020
  • Published Date: October 31, 2020
    Graphical Abstract
    • Abstract
  • In this study, the distribution of tomato spotted wilt virus(TSWV) in vascular tissues of systemic host tobacco K326 was observed by transmission electron microscopy (TEM) to analyze the long-distance transportation of TSWV. The results showed that vesicle-enveloped viral particles and nuclear capsid aggregates (NCA) were found in vessel elements. Vesicle-enveloped viral particles were found in vascular parenchyma cells, and there were more vesicles in cytoplasm than the CK. Vesicle-enveloped viral particles were found in companion cells, and a large number of vesicles with a diameter of about 120 nm were found in cytoplasm. At the same time, pathological changes in mitochondria were also found, including the expansion and disappearance of the mitochondrial stroma lamella. There was no viral particles in the sieve element, but vesicles with a diameter of about 80-200 nm were found in the sieve element. Our finding suggested that the TSWV virions are likely transported by xylem, and the TSWV vesicle-related complexes (VRC) may be transported by phloem.
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  • Whitfield A E, Ullman D E, German T L. Tospovirus-thrips interactions [J]. Annual Review of Phytopathology, 2005, 43(1): 459−489. DOI: 10.1146/annurev.phyto.43.040204.140017
    Best R J. Tomato spotted wilt virus [J]. Advances in Virus Research, 1968, 13: 65−146. DOI: 10.1016/S0065-3527(08)60251-1
    郑宽瑜, 吴阔, 董家红, 等. 番茄斑萎病毒对云南莴苣类蔬菜的侵染危害 [J]. 植物保护, 2015, 41(5): 174−178. DOI: 10.3969/j.issn.0529-1542.2015.05.033
    Oliver J E, Whitfield A E. Thegenus Tospovirus: emerging Bunyaviruses that threaten food security [J]. Annual Review of Virology, 2016, 3(1): 101−124. DOI: 10.1146/annurev-virology-100114-055036
    Tsompana M, Abad J, Purugganan M, et al. The molecular population of the Tomato spotted wilt virus (TSWV) genome [J]. Molecular Ecology, 2005, 14(1): 53−66.
    Hipper C, Brault V, Ziegler-Graff V, et al. Viral and cellular factors involved in phloem transport of plant viruses [J]. Frontiers in Plant Science, 2013, 4: 1−24.
    Opalka N, Brugidou C, Bonneau C, et al. Movement of rice yellow mottle virus between xylem cells through pit membranes [J]. Proceedings of the National Academy of Sciences of the United States of America, 1998, 95(6): 3323−3328. DOI: 10.1073/pnas.95.6.3323
    Moreno I M, Thompson J R, García-Arenal F. Analysis of the systemic colonization of cucumber plants by Cucumber green mottle mosaic virus [J]. Journal of General Virology, 2004, 85(3): 749−759. DOI: 10.1099/vir.0.19540-0
    Manabayeva S A, Shamekova M, Park J W, et al. Differential requirements for Tombusvirus coat protein and P19 in plants following leaf versus root inoculation [J]. Virology, 2013, 439(2): 89−96. DOI: 10.1016/j.virol.2013.01.011
    Fuentes A L, Hamilton R I. Failure of long-distance movement of southern bean mosaic virus in a resistant host is correlated with lack of normal virion formation [J]. Journal of General Virology, 1993, 74(9): 1903−1910. DOI: 10.1099/0022-1317-74-9-1903
    Hipper C, Monsion B, Bortolamiol-Bécet D, et al. Formation of virions is strictly required for turnip yellows virus long-distance movement in plants [J]. Journal of General Virology, 2014, 95(2): 496−505. DOI: 10.1099/vir.0.058867-0
    Grangeon R, Jiang J, Wan J, et al. 6K2-induced vesicles can move cell to cell during turnip mosaic virus infection [J]. Frontiers in Microbiology, 2013, 4: 351.
    Taliansky M, Roberts I M, Kalinina N, et al. An umbraviral protein, involved in long-distance RNA movement, binds viral RNA and forms unique, protective ribonucleoprotein complexes [J]. Journal of Virology, 2003, 77(5): 3031−3040. DOI: 10.1128/JVI.77.5.3031-3040.2003
    Wan J, Cabanillas D G, Zheng H, et al. Turnip mosaic virus moves systemically through both phloem and xylem as membrane-associated complexes [J]. Plant Physiology, 2015, 167(4): 1374−1388. DOI: 10.1104/pp.15.00097
    Wan J, Laliberté J F. Membrane-associated virus replication complexes locate to plant conducting tubes [J]. Plant Signaling & Behavior, 2015, 10(8): e1042639. DOI: 10.1080/15592324.2015.1042639
    Lewandowski D J, Adkins S. The tubule-forming NSm protein from Tomato spotted wilt virus complements cell-to-cell and long-distance movement of Tobacco mosaic virus hybrids [J]. Virology, 2005, 342(1): 26−37. DOI: 10.1016/j.virol.2005.06.050
    Zhang Y, Zhang C, Li W. The nucleocapsid protein of an enveloped plant virus, tomato spotted wilt virus, facilitates long-distance movement of Tobacco mosaic virus hybrids [J]. Virus Research, 2012, 163(1): 246−253. DOI: 10.1016/j.virusres.2011.10.006
    Li W, Lewandowski D J, Hilf M E, et al. Identification of domains of the Tomato spotted wilt virus NSm protein involved intubule formation, movement and symptomatology [J]. Virology, 2009, 390(1): 110−121. DOI: 10.1016/j.virol.2009.04.027
    方琦, 丁铭, 董家红, 等. 云南澳洲坚果苗木感染番茄斑萎病毒属病毒初报 [J]. 园艺学报, 2013, 40(2): 350−354.
    Ham B K, Lucas W J. Phloem-mobile RNAs as systemic signaling agents [J]. Annual Review of Plant Biology, 2017, 68(1): 173−195. DOI: 10.1146/annurev-arplant-042916-041139
    Singh P, Indi S S, Savithri H S. Groundnut bud necrosis virus encoded NSm associates with membranes via its C-terminal domain [J]. PLoS One, 2014, 9(6): e99370. DOI: 10.1371/journal.pone.0099370
    Singh P, Savithri H S. GBNV encoded movement protein (NSm) remodels ER network via C-terminal coiled coil domain [J]. Virology, 2015, 482: 133−146. DOI: 10.1016/j.virol.2015.01.030
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