Somatic Embryogenesis and Plantlet Regeneration of Akebia trifoliate

The young embryos of the mature seed of Akebia trifoliate were used to study the effect of different concentrations of plant growth regulators: 2, 4−D (1.0, 2.0, 4.0 mg/L), NAA (0.05, 0.1, 0.2, 0.5, 1.0 mg/L), 6−BA (0.1, 0.2, 0.5, 1.0 mg/L), KT (0.2, 0.5, 1.0 mg/L) on callus induction and somatic embryogenesis of A. trifoliate. And select the medium for inducing callus, somatic embryo, proliferation of secondary embryos and plant regeneration. The system of embryogenesis and plant regeneration of A. trifoliate was established through indirect induction. The results show that the optimal sterilization treatment was 0.1 % HgCl₂ for 18 min, washed with sterile water and inoculated on callus induction medium supplemented with 0.05 % PPM; the optimal medium for callus induction from immature embryos of A. trifoliate was MS supplemented with 2.0 mg/L 2, 4-D+0.2 mg/L NAA, and the callus induction rate was 95.67 %. The optimal medium for somatic embryogenesis was MS supplemented with 0.1 mg/L NAA+0.2 mg/L 6−BA, and the somatic embryo induction rate was 55%. The optimal medium for the proliferation of secondary embryos was MS supplemented with 0.05 mg/L NAA+0.1 mg/L 6-BA, and the proliferation ratio reached 19.84. Somatic embryos differentiated gradually with the increase of subculture times and the proliferation activity of secondary embryos became weak. Part of mature somatic embryos can be regenerated into seedlings after differentiation, the regenerated plants can grow strong in MS, and they were transferred to an artificial light incubator for 3–5 days, and then transferred to a 2∶1∶1 matrix of peat: vermiculite: perlite. The roots were stable and the morphology was normal.