Influence of Volatiles from Leaves of Celtis kunmingensis and Alnus nepalensis on Gene Expression of Vitellogenin and Its Receptor of Tomicus yunnanensis

In order to reveal the influence of volatiles from leaves of the non-host plants including Celtis kunmingensis and Alnus nepalensis on gene expression of the vitellogenin(Vg) and its receptor(VgR) of Tomicus yunnanensis, the transcriptions of these 2 genes at different development stages(egg, larvae, pupae and adult) and in different tissues of female adults(head, thorax, wing, ovary and abdomen carcass), and in response to the leaf volatiles from 2 non-host plants were determined using fluorescence quantitative real-time PCR. The results showed that the expressions of Vg and VgR genes are significant higher in pupae and adult than that in other developmental stages, and they were expressed significantly higher in adult than in pupae. Vg gene was expressed highest in abdomen carcass, while VgR gene has abundantly ovary-specific expression. The expression of Vg gene significantly reduced by the treatment of leaf volatiles from C. kunmingensis after 20 d and 40 d, while its expression significantly upregulated after 60 d. After the treatment of leaf volatiles from A. nepalensis 20 d and 40 d, the gene expression of VgR was significantly downregulated, while the expression of this gene didn't change after 60 d. The findings imply that egg production of T. yunnanensis can be influenced by non-host volatiles via regulation of its Vg and VgR gene expression, which lay a foundation for further revealing the underlying mechanisms.