Metabolomics of Vetiveria zizanioides Leaves Under Prometryn Stress

To study the metabolic response mechanism of Vetiveria zizanioides under prometryne stress, extensive targeting metabolism was adopted to study the changes of metabolites in the leaves of V. zizanioides under 5 mg/L prometryne stress. The results showed that when prometryne was stressed for 5 days, the total amount of differential metabolites was 121 compared with the control; compared with the control and stress for 5 days, the total amount of differential metabolites of 10 days stress was 151 and 48 respectively. In the process of stress, flavonoids, alkaloids and lipids are the most down-regulated metabolites, and nucleotides and their derivatives, alkaloids and others(such as microorganisms, sugars and alcohols) are the most up-regulated metabolites; the estimation of pyruvate,jasmonic acid, serotonin and other substances is critical in the stress resistance process, in which jasmonic acid may play a role in the termination of defense signal in the later stage of stress; the amount of down-regulated free amino acids was higher than that of up-regulated free amino acids. Analysis on metabolic pathway revealed that arginine and proline metabolism and plant hormone signal transduction play a major role in leaf resistance to prometryne stress.