Construction of Callus Regeneration System in Grape Hyacinth

The callus induction and plant regeneration of grape hyacinth was carried out, utilizing the flower buds and leaves of Muscari armeniacum as explants. The results show that the best medium for callus induction and proliferation was MS + 1.0‍ mg/L^‍ 2,4-D + 0.1 mg/L 6-BA, and the callus induction rate was 100%. After 35 days of subculture, there was no significant difference on callus proliferation in different medium formulations. Meanwhile, liquid culture and light culture were beneficial to the proliferation of calli. After 4 cycles of the subculture of 1 g M. armeniacum flower and leaf-derived callus, the maximum average fresh weight was 10.36 ± 1.13 g and 10.55 ± 2.29 g. The results showed that different media formulations had no significant effect on flower and leaf-derived callus proliferation in M. armeniacum, and both liquid culture and light culture were beneficial to callus proliferation. Furthermore, embryogenic and non-embryogenic callus could be regenerated to plants by somatic embryogenesis and organogenesis, respectively. The regeneration rates were all 100%.