Effects of Combined Inoculation of Endophytic Fungi on the Growth and Nutrient Content of <i>Tripterygium wilfordii</i>

Feng Liyuan; Hu Qingxia; Song Ping; Lin Zhaoshou; Feng Lei

doi:10.11929/j.swfu.202312025

Journal of Southwest Forestry University > 2024 > 44(6): 63-71. > DOI: 10.11929/j.swfu.202312025

Feng Liyuan, Hu Qingxia, Song Ping, Lin Zhaoshou, Feng Lei. Effects of Combined Inoculation of Endophytic Fungi on the Growth and Nutrient Content of Tripterygium wilfordii[J]. Journal of Southwest Forestry University, 2024, 44(6): 63-71. DOI: 10.11929/j.swfu.202312025

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Effects of Combined Inoculation of Endophytic Fungi on the Growth and Nutrient Content of Tripterygium wilfordii

1.
College of Forestry, Fujian Agriculture and Forestry University, Fuzhou Fujian 350007, China
2.
College of Juncao Science and Ecology, Fujian Agriculture and Forestry University, Fuzhou Fujian 350007, China
3.
Fujian Provincial Key Laboratory of Forest Ecosystem Processing and Management, Fuzhou Fujian 350002, China
4.
Datian Taoyuan State-owned Forest Farm in Fujian Province, Sanming Fujian 366031, China
5.
College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou Fujian 350002, China

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Received Date: December 12, 2023
Accepted Date: April 23, 2024
Available Online: April 29, 2024

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Abstract

Abstract

To investigate the effects of combined inoculation of endophytic fungi on the growth and nutrient content of host plants, 4 strains of endophytic fungi, Penicillium steckii NS6, Meyerozyma guilliermondii NS31, Hypocrea nigricans NS32 and Fusarium oxysporum NS33, were paired and inoculated into micropropagated Tripterygium wilfordii seedlings. Sterile PDA medium plugs were served as controls. The growth, chlorophyll content and nutrient content(C, N, P and K) of the micropropagated seedlings were measured. The results indicated that combined inoculation of endophytic fungi significantly enhanced the height of micropropagated seedlings. Specially, the height under NS6 + NS33 and NS6 + NS32 inoculation was 2.72 and 2.28 times greater than the control, respectively. Additionally, the root length and dry mass under NS6 + NS32 inoculation were significantly higher compared to the control. The dry mass under NS6 + NS33 inoculation was significantly higher compared to the control. The combined inoculation of NS6 + NS32 had synergistic effects on increasing the mass of the micropropagated seedlings, surpassing the effects of single inoculation. Furthermore, inoculation of NS6 + NS32 and NS6 + NS33 significantly elevated chlorophyll a, b, and a + b levels. The combined inoculation of each fungal strain increased the C and N content compared to the control. However, there was no significant effect on P and K levels. The influence of endophytic fungi on the growth and nutrient content of micropropagated T. wilfordii seedlings varied depending on the combination of fungal species. Notably, the combination of NS6 and NS32 strains exhibited enhanced growth promotion effects.
- Tripterygium wilfordii,
- endophytic fungi,
- inoculation,
- growth promotion,
- nutrient

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References (44)

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Effects of Combined Inoculation of Endophytic Fungi on the Growth and Nutrient Content of Tripterygium wilfordii

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