Screening and Identification of Ammonia-removing Bacteria in the Feces of Protaetia brevitarsis Larvae

To isolate and screen microorganisms with ammonia removal effect from the feces of the larvae of Protaetia brevitarsis, and to provide microbial resources for the conversion and utilization of organic waste, 2 strains of ammonia-removing bacteria N1 and N2 were screened from the feces of the larvae of P. brevitarsis by means of enrichment and domestication, preliminary screening and re-screening. The strains were identified by morphological, physiological and biochemical, and 16SrDNA molecular identification. The physiological characteristics and growth curve of the strain were determined. The effects of single strain and compound strain on NH₃ release within 25 days were determined by boric acid absorption Kjeldahl method. The findings indicated that both N1 and N2 showed obvious ammonia removal ability, and the ammonia removal effect of the compound strain was higher than that of the single strain. N1 was identified as Ochrobactrum tritici, and N2 was identified as Ochrobactrum sp. The growth curves of N1 and N2 had their own characteristics. The 2 strains grew best at 30 ℃–37 ℃ pH = 5–9. The 2 strains isolated from the larvae of P. brevitarsis can effectively reduce the release of NH₃, and the ammonia removal effect of the 2 strains is higher than that of the single strain, which can be used as an effective ammonia removal microorganism during the fermentation of organic waste.