Prediction and Analysis of Genome Size of <i>Orophea yunnanensis</i>

Yuan Xiaolong; Yang Yuexian; Mao meihua; Zhang Chuanguang; Wang Yi

doi:10.11929/j.swfu.202407030

Journal of Southwest Forestry University > 2025 > Corrected proof > DOI: 10.11929/j.swfu.202407030

Yuan X L, Yang Y X, Mao M H, et al. Prediction and Analysis of Genome Size of Orophea yunnanensis[J]. Journal of Southwest Forestry University, 2025, 45(3): 1–6. DOI: 10.11929/j.swfu.202407030

Citation:

Yuan X L, Yang Y X, Mao M H, et al. Prediction and Analysis of Genome Size of Orophea yunnanensis[J]. Journal of Southwest Forestry University, 2025, 45(3): 1–6. DOI: 10.11929/j.swfu.202407030

Citation:

Yuan X L, Yang Y X, Mao M H, et al. Prediction and Analysis of Genome Size of Orophea yunnanensis[J]. Journal of Southwest Forestry University, 2025, 45(3): 1–6. DOI: 10.11929/j.swfu.202407030

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Prediction and Analysis of Genome Size of Orophea yunnanensis

1.
Yunnan Forestry Technological College, College of Landscape and Horticulture, Kunming, 650224
2.
Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants, Conservation of Rare, Endangered & Endemic Forest Plants, Public Key Laboratory of the State Forestry Administration, Yunnan Academy of Forestry and Grassland, Kunming, 650201

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Received Date: July 16, 2024
Revised Date: September 22, 2024
Accepted Date: October 11, 2024
Available Online: October 16, 2024

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Abstract

Abstract

Flow cytometry and K-mer survey methods were utilized to estimate the genome size of Orophea yunnanensis, and analyzed the genome and its characteristics, and Zea mays DNA was used as standards in this study. The results showed that, the genome size of O. yunnanensis was estimated to be approximately 866.53 Mb or 801.96 Mb by flow cytometry; 125.97 Gb original data was obtained through high-throughout sequencing, and approximately high-quality 123.33 Gb clean data was acquired after processing, and this illustrated the sequencing data was good; the genome size of O. yunnanensis was about 782.9 Mb(K-mer=19), the heterozygous rate was about 0.86 %; when K-mer=21, the genome size was estimated to be 782.20 Mb, and the heterozygous rate was about 0.90 %; these suggested the genome of O. yunnanensis was highly heterozygotic genome; the top three species Chieniodendron hainanense, Alphonsea hainanensis and Stelechocarpus burahol were found to be the same family as O. yunnanensis compared using NT database.
- Orophea yunnanensis,
- flow cytometry,
- genome survey analysis,
- genome size

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References (31)

References

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