Tag A Regulation of CYP724B1 to Inhibite Tomato Spotted WiltOrthotospovirus NSs Gene Replication

Tagitinin A (Tag A) can inhibit the infection of Tomato spotted wilt orthotospovirus (TSWV). RT-qPCR was used to further verify the data and found that the expression of Nitab4.5_0007569g0010(CYP724B1) gene was differently significant increased after Tag A treatment. Using virus-induced gene silencing (VIGS) technology, the CYP724B1 gene was silenced, followed by preventive experimental treatment. The findings indicated that after CYP724B1 gene was overexpressed, the expression of the NSm and NSs genes was significantly decreased compared to the control in the inoculated and systemic leaves. These results indicate that Tag A could regulate the CYP724B1 gene to inhibit the expression of NSm and NSs to defense against TSWV infection.