Analysis of Genome Size of Nuphar pumila

In order to explore the the genome size and characteristics of the Nuphar pumila (Timm) DC, this study selected three N. pumila. samples numbered: KMPPC01, KMPPC02, and KMPPC03 for flow cytometry detection. The KMPPC01 was selected for high-throughput sequencing. Combined with bioinformatics methods such as K-mer analysis, conducted preliminary research on the genome of N. pumila. The results are as follows: (1) The genome size of N. pumila was determined to be approximately 2187.12 Mb by flow cytometry and the converted C value was approximately 1.12 pg; (2) The raw data obtained by high-throughput sequencing was 111.76 Gb, and the clean data obtained after filtering was 108.39 Gb; (3) K-mer analysis showed that the genome size of N. pumila was 2094.71 Mb, the GC content was 38.20%, the heterozygosity was 1.89%, the proportion of repeated sequences was 62.63%. In summary, the genome of N. pumila. is a highly heterozygous and highly repetitive complex genome with a size of approximately 2094.71 Mb～2187.12 Mb.