Analysis of lignocellulose and essential oil components in branches and leaves of Cinnamomum kanahirae at different ages

To alleviate the dependence of Taiwanofungus camphorata cultivation on Cinnamomum kanehirae (CK) logs older than 10 years, we systematically evaluated the physicochemical traits and essential oil profiles of young CK branches and leaves to explore their feasibility as substitutes. Leaves and branches were harvested from three trees at each age (5, 8 and 10 years). The contents of cellulose, hemicellulose, lignin, soluble sugar, starch and alkaloids were quantified. The essential oil yield and components were analyzed by steam distillation coupled with GC/MS. The soluble sugar content in the leaves of 5- and 8-year-old CK trees was significantly higher than that of 10-year-old trees, while the starch content was significantly lower. No significant differences in lignin content were observed among the branches of different ages. The essential oil yields of the leaves of 5-year-old CK trees was1.5%, which were significantly higher than that of 10-year-old trees (0.8%) (P=0.0455). The cellulose and hemicellulose contents were slightly higher in young tissues, while the lignin content was similar. The alkaloid content could be readily reduced by water extraction. Thus, 5-8-year-old CK branches and leaves already possess the major carbon skeleton components and oil productivity required to replace 10-year-old logs. Subsequent adjustments to soluble sugar, starch, and alkaloids levels via water soaking or enzymatic treatment can further approximate the nutritional environment of 10-year-old wood, offering new feedstock for short-cycle T. camphorata cultivation.