Preliminary Study on Adjuvant Selection for a Biocontrol Trichoderma atroviride Power Formulation

Based on the preliminary studies on the biological compatibility of Trichoderma atroviride (TR1) with different types of commonly used carriers and wettable power adjuvants, the stabilizers, UV protection agents and hydrogels with appropriate biological compatibility with TR1 were screened by means of measuring the hypha growth rate and the spore germination rate. The results showed that mycelium growth of TR1 was not significantly influenced by CMC-Na stabilizers, and TR1 spore germination rate in the culture medium with low concentration of CMC-Na stabilizer was not significantly different from the control. Among five types of the UV protection agents. Both dextrin and xanthan gum showed favorable biological compatibility to TR1, which did not have much influence upon the growth of TR1 mycelium, either. It was showed that the hydrogel tested did not impact the growth of TR1 mycelium, it could also provide the TR1 mycelium growth with carbon source when there was in case a short of carbon in the culture medium. The relative humidity had little impact on TR1 spore germination on the culture medium with hydrogel. The study results provided the wettable powder formulation research development for TR1 with theoretical foundation.