Molecular Detection of Inonotus weirii Gilgertson

Inonotus weirii is an important plant quarantine pathogen in China. In order to set up an accurate,sensitive and quick way to detect this pathogen,a pair of specific primers,i.e.,IW1,IW2 were designed on the basis of the differences in the internal transcribed spacer(ITS) sequences between I.weirii and four species in the genus Inonotus,which were mostly close to Inonotus weirii in terms of morphology. The results of PCR showed that a 165bp product was only obtained from the sample of I.weirii. The detection sensitivity was 100 pg genomic DNA per 25μL PCR reaction volume.In addition,IW1,IW2 primers were successfully applied to realtime PCR with higher sensitivity of 0.1 pg genomic DNA per 20μL PCR reaction volume.The I.weirii pathogen could be rapidly and accurately detected from artificially blurred wood by these two methods.