KANG Liu, XIONG Zhi, DU Jianwei, LIU Shaoxiong, ZHU Lili. Isolation of Cellulose Degradating Bacteria from Bark BeetlesIntestine and Enzyme Production Properties[J]. Journal of Southwest Forestry University, 2012, 32(2): 7-10. DOI: 10.3969/j.issn.2095-1914.2012.02.002
Citation: KANG Liu, XIONG Zhi, DU Jianwei, LIU Shaoxiong, ZHU Lili. Isolation of Cellulose Degradating Bacteria from Bark BeetlesIntestine and Enzyme Production Properties[J]. Journal of Southwest Forestry University, 2012, 32(2): 7-10. DOI: 10.3969/j.issn.2095-1914.2012.02.002
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Isolation of Cellulose Degradating Bacteria from Bark BeetlesIntestine and Enzyme Production Properties

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  • Five strains of cellulose degrading bacteria were isolated from the intestine of young bark beetle, and the activity of cellulase generated by these strains was determined by Dus cellulose selective medium on filter paper or absorbent cotton with CMC-Na as fermentation substrate. The results showed that HS-11-5 was the strain with the highest cellulase activity (32.62 U/mL), whose optimum cellulase producing conditions were as follows: 50h of fermentation; (NH4)2SO4 as nitrogen source; under pH value of 7.5-8.5 at 28-30℃; and 12%-16% as the medium loading proportion.
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