Cloning and Sequence Character Analysis of FullLength cDNA of Cellulose Synthase PuCesA6 from Populus ussuriensis

The total RNA extracted from Populus ussuriensis leaves was used for RT-PCR, then the fulllength cDNA sequence of cellulose synthase gene (PuCesA6) was amplified. The sequence analysis showed that the fulllength cDNA of PuCesA6 consisted of 3778 bp, with an ORF of 3264 bp encoding 1087 amino acids. The amino acid sequences of the PuCesA6 showed characteristics of cellulose synthase gene of the angiospermous plant species, i.e., it contained a ringtype zincfinger domain, followed by a hyper variable region I (HVRI) and a hyper variable region II (HVRII), a conserved domain related to βglycoside transferase and 8 transmembrane domains. Homology analysis of the deduced amino acid showed 89% identity to BlCesA5 from Betula luminifera and 80% identity to ZmCesA8 from Zea mays. The sequence analyses results also showed that the ringtype zincfinger domain of 8 types of cellulose synthase gene CesA and the amino acid sequences at Cterminal domain were highly conservative.