Construction and Analysis of Enriched Microsatellite Library of Tomicus brevipilosus

The enriched microsatellite library of Tomicus brevipilosus (Eggers) was constructed using biotinmagnetic bead enrichment method. One hundred clones randomly chosen from the library were sequenced. The results showed that 91% of microsatellite loci were obtained. Among which the microsatellite sequences with more than 10 times of alkaline base repetitions accounted for 36%, and the highest alkaline base repetition was 63 times. From these microsatellites, 47.3% (about 43 repeat motifs) were perfect, 24.2% (about 22 repeat motifs) were imperfect, and 16.5% (about 15 repeat motifs) were compound. The results indicated that the enriched microsatellite library was of highquality, in which the proportion of perfect microsatellites types was higher than that of the imperfect and compound types, which was in accordance with the feature of microsatellite sequences of other eukaryote.