Bioinformatics Analysis of CHI Genes in Eight Plant Species

The residual amino acid sequences of CHI of Arabidopsis thaliana, Pyrus pyrifolia cultivar Meirensu, Malus hybrid cultivar, Prunus persica ,Vitis vinifera, Fragaria×ananassa, Citrus sinensis, Zea mays were adopted from GeneBank. The composition of the residual amino acid sequences, leader peptide, signal peptide, transmembrane topological structure, hydrophobicity / hydrophilicity, the secondary structure, the tertiary structure and functional domains of the proteins were predicted and analyzed. The results showed that CHI of 8 plant species was a nontransmembrane protein without leader peptide and signal peptide. It was supposed that CHI was positioned to the cell matrix to function directly, the polypeptide chain was generally hydrophobic. There were 4 types of secondary structure including αhelix, βturn, random coil and extended stand. Except for that the irregular coil was the main secondary motif of Prunus persica, αhelix was the most principal structure motif of the rest 7 plant species. All the eight plant species possessed a chalcone isomerase active site.