Effects of Antibiotic on Differentiation and Regeneration of Different Petunia hybride Genotypes

The effect of Kanamycin (Kan) and Cefotaxime (Cef) with different concentrations on adventitious bud differentiation from leaflets of Petunia hybride and rooting of the tube plantlets was studied by taking 4 cultivars, namely ‘Meishengchenguang’, ‘Huangjinrouhong’,‘Shanhuhong’ and ‘Huangjinziluolanxingtiao’ as experimental materials. The appropriate antibiotic concentrations were screened. The results showed that 30mg/L Kan could restrain adventitious bud differentiation, and no adventitious buds occurred when the concentration of Kan was 50mg/L.Similarly, low concentration of Kan restrained the rooting of tube plantlets, while no adventitious roots took place when Kan concentration was 30mg/L. The occurrence of adventitious buds were limited under the condition of 550mg/L Cef, and 400mg/L Cef was the suitable concentration for adventitious bud differentiation.