Abstract: Glyphosate is a high-active, low-toxic, less-persistent, systematic-trans-locatable and sterilant herbicide. So far, it has been widely applied in teagardening, fruit-gardoning and so on. The paper reports on an experiment in which glyphosate is used in tea-gardening. The result shows:1.Adoption of reasonable dose and proper usage, glyphosate has no bad effects on tea-growth and tea quality. 2.The pure-glyphosate dose of controlling sensitive-weeds is 450 g/ha-750 g/ha; of medium-resistant-weeds in 900 g/ha-1350 g/ha; of strong-resistant-weeds is 1650 g/ha-2250 g/ha If add 750 g/ha-1500 g/ha washing-powder into medicine liquid, a higher medical efficiency will be obtained. 3.The application of glyphosate, the cost of management can be reduced. At all events, controlling weeds in tea-gardening with glyphosate is able to achieve high-active, economic success, and has no bad effect for tea, and it is worthy of being widely applied.