孙敏, 汤绍虎, 陈安和, 杨兰英, 王必浓. 银杉成熟胚离体培养及愈伤组织诱导的研究[J]. 西南林业大学学报, 1994, 14(3): 155-158. DOI: 10.11929/j.issn.2095-1914.1994.03.004
引用本文: 孙敏, 汤绍虎, 陈安和, 杨兰英, 王必浓. 银杉成熟胚离体培养及愈伤组织诱导的研究[J]. 西南林业大学学报, 1994, 14(3): 155-158. DOI: 10.11929/j.issn.2095-1914.1994.03.004
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Sun Min, Tang Shaohu, Chen Anhuo, Yang Lanying, wang Binong. STUDIES ON THE GROWTH OF MATURE EMBRYO OF CATHAY A ARGYROPHYLLA IN VITRO AND INDUCING OF CALLUS[J]. Journal of Southwest Forestry University, 1994, 14(3): 155-158. DOI: 10.11929/j.issn.2095-1914.1994.03.004
Citation: Sun Min, Tang Shaohu, Chen Anhuo, Yang Lanying, wang Binong. STUDIES ON THE GROWTH OF MATURE EMBRYO OF CATHAY A ARGYROPHYLLA IN VITRO AND INDUCING OF CALLUS[J]. Journal of Southwest Forestry University, 1994, 14(3): 155-158. DOI: 10.11929/j.issn.2095-1914.1994.03.004
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银杉成熟胚离体培养及愈伤组织诱导的研究

STUDIES ON THE GROWTH OF MATURE EMBRYO OF CATHAY A ARGYROPHYLLA IN VITRO AND INDUCING OF CALLUS

    摘要
  • 摘要: 将银杉种子的成熟胚剥离出来,在LP+NAA1.5×10-6培养基中离休培养,成苗率为87%。以无菌幼苗的子叶、下胚轴、顶芽作为外植体,在LP附加KT0.5,BA0.5,NAAl×10-6的培养基中诱导出愈伤组织。这一研究为银杉离体胚培养进行快速繁殖,作了有益的尝试。

     

    Abstract: The mature embryos of Cathaya argyrophylla Chun et Kuang were removed from seeds and cultured on LP+NAA 0.5~2.0×10-6 media. The rate of seedling is 87% on the medium contained NAA 1.5×10-6. The cutting segments of sterile cotyledon, apical bud and hypocotyl were cultured on LP medium contained KT 0.5. BA 0.5. NAA 1×10-6 and the callus was formed after 3 weeks.

     

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