胡杨膜系统的盐稳定性及盐胁迫下的代谢调节
The Stability of the Membrane and Metabolic Response of P. euphratica Under Salt Stress
-
摘要: 比较胡杨和群众杨在盐分胁迫下的生化反应发现:(1)胡杨的细胞膜系统能忍耐200 mM NaCl的高盐处理,其膜透性在高盐处理3 d后没有显著增加,而群众杨的膜透性随盐分浓度的升高而升高.胡杨的SOD酶活性上升较快,而且随盐胁迫强度的加强而增加,群众杨受盐胁迫SOD酶活性上升较慢,且强盐胁迫下活性反而减弱,说明群众杨SOD酶活性本身的盐稳定性差,不能有效保护膜系统.盐胁迫蛋白电泳谱带也有相似的变化趋势:群众杨的谱带由10条减少到3条,胡杨则由4条增加到7条.说明胡杨在盐胁迫下出现了稳定膜结构并合成可兼容溶质有关的蛋白.(2)胡杨有较强的代谢调节能力.盐处理后胡杨和群众杨的脯氨酸和精胺的增加水平没有显著差异,差异主要是腐胺含量:低盐胁迫7 d,胡杨叶的腐胺含量是群众杨的3.4倍,高盐胁迫7 d则是4.4倍.多胺,特别是其中的腐胺能稳定生物大分子和保护细胞膜的完整性.因此,可以认为在盐胁迫条件下大量增加腐胺的生物合成是胡杨抗盐机理的一部分,可以作为树木抗盐的一种生理指标.Abstract: The comparison of biochemical response between P. euphratica and P. popularis showed that plasmmnan of P. euphratica could tolerate high salt stress of 200 mM NaCl The membrane permeability of P. euphratica did not increase significantly by day 3 after salt treatment,while that of hybrid increased with the increase of salt treatments The factors related to the high membrane stability in P. euphratica might be:(1)SOD activity increased with increasing salt treatments;(2)the synthesis of compatible osmotic solutes such as polyamine and proline was sensitive to salt stress In addition,the new bands proteins appeared under high salt stress,which was also an indicator of salt tolerance for P. euphratica.