Abstract: Selection of explants and culture mediums and methods of tissue culture in Betula alnoides are studied.Regenerated plants from tissue culture are obtained.Based on the above study, program of callus initiation and forming embryoid during tissue culture are analyzed.Meanwhile, affecting factors of Tissue culture to Betula alnoides also are analyzed as follows:The explant selected either in spring or in autumn are the easiest to produce callus;the bigger the ratio of 6BA to NAA is, the more adventitious buds are;the embryoid could be induced either in dark or light conditions from embryoid callus;the most suitable medium for producing the somatic embryoid differention is the MS medium which contains 5mg/L ABA and 20g/L sucrose.